Basic Information

Strain name
C57BL/6-Ccr9tm1(CCR9)/Bcgen
Common Name
B-hCCR9 mice
Background
C57BL/6
Catalog Number
110972
Aliases
GPR28; CDw199; GPR-9-6; CC-CKR-9
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCR9 mice. The mouse Ccr9 gene that encode the full length of coding region were replaced by human CCR9 coding sequence in B-hCCR9 mice.

Protein expression analysis

Strain specific CCR9 expression analysis in homozygous B-hCCR9 mice by flow cytometry. Thymocytes were collected from WT and homozygous B-hCCR9 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR9 antibody. Mouse CCR9 was detectable in wild-type C57BL/6 mice (+/+). Human CCR9 was exclusively detectable in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.

mRNA expression analysis

Strain specific analysis of CCR9 gene expression in wild-type mice and hCCR9 mice by RT-PCR. Mouse Ccr9 mRNA was detectable only in thymocytes of wild-type mice (+/+). Human CCR9 mRNA was detectable only in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.

Summary

mRNA expression analysis:

Human CCR9 mRNA was detectable in B-hCCR9 mice (H/H) mice but not in wild-type mice.

Protein expression analysis:

Human CCR9 was exclusively detectable in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.