Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCR9 mice. The mouse Ccr9 gene that encode the full length of coding region were replaced by human CCR9 coding sequence in B-hCCR9 mice.
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Protein expression analysis
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Strain specific CCR9 expression analysis in homozygous B-hCCR9 mice by flow cytometry. Thymocytes were collected from WT and homozygous B-hCCR9 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR9 antibody. Mouse CCR9 was detectable in wild-type C57BL/6 mice (+/+). Human CCR9 was exclusively detectable in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.
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mRNA expression analysis
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Strain specific analysis of CCR9 gene expression in wild-type mice and hCCR9 mice by RT-PCR. Mouse Ccr9 mRNA was detectable only in thymocytes of wild-type mice (+/+). Human CCR9 mRNA was detectable only in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.
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Summary
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mRNA expression analysis:
Human CCR9 mRNA was detectable in B-hCCR9 mice (H/H) mice but not in wild-type mice.
Protein expression analysis:
Human CCR9 was exclusively detectable in homozygous B-hCCR9 mice (H/H) but not in wild-type C57BL/6 mice.
