mRNA and protein expression analysis
Species-specific CGRP expression analysis in wild-type and B-hCGRP mice by RT-PCR and western blot. (A) mRNA expression. Due to high homology, sequencing were performed to verify whether humanization was successful (right). RT-PCR and sequencing results showed that murine Cgrp mRNA was detected in the brain from wild-type C57BL/6 (+/+) mice, while human CGRP mRNA was exclusively detected B-hCGRP (H/H) mice. (B) Protein expression. Heart was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCGRP (H/H) mice, and analyzed by western blot with a known cross-reactive anti-CGRP antibody. The CGRP protein can be detected in wild-type and B-hCGRP mice.