Basic Information

Strain name
C57BL/6-Cldn6tm1(CLDN6)/Bcgen
Common name
B-hCLDN6 mice
Background
C57BL/6
Catalog number
111086
NCBI Gene ID

Targeting Strategy

Gene targeting strategy for B-hCLDN6 mice

The full encoding sequence region of mouse CLDN6 gene was replaced by the counterpart gene sequence of human CLDN6 in B-hCLDN6 mice.

Protein Expression Analysis

Strain specific CLDN6 expression analysis in heterozygous B-hCLDN6 mice by western blot. Liver tissue was collected from wild-type C57BL/6 (+/+) and heterozygous B-hCLDN6 mice (H/+) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice and heterozygous B-hCLDN6 mice. Human CLDN6 was exclusively detectable in heterozygous B-hCLDN6 mice but not in wild-type mice.

Strain specific CLDN6 expression analysis in homozygous B-hCLDN6 mice by western blot. Liver tissue and thymus were collected from wild-type C57BL/6 (+/+) and homozygous B-hCLDN6 mice (H/H) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice. Human CLDN6 was exclusively detectable in homozygous B-hCLDN6 mice but not in wild-type mice.