Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hCLDN6 mice
The full encoding sequence region of mouse CLDN6 gene was replaced by the counterpart gene sequence of human CLDN6 in B-hCLDN6 mice.
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Protein Expression Analysis
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Strain specific CLDN6 expression analysis in heterozygous B-hCLDN6 mice by western blot. Liver tissue was collected from wild-type C57BL/6 (+/+) and heterozygous B-hCLDN6 mice (H/+) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice and heterozygous B-hCLDN6 mice. Human CLDN6 was exclusively detectable in heterozygous B-hCLDN6 mice but not in wild-type mice.
Strain specific CLDN6 expression analysis in homozygous B-hCLDN6 mice by western blot. Liver tissue and thymus were collected from wild-type C57BL/6 (+/+) and homozygous B-hCLDN6 mice (H/H) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice. Human CLDN6 was exclusively detectable in homozygous B-hCLDN6 mice but not in wild-type mice.
