Basic Information

Strain name
C57BL/6-Cx3cr1tm1(CX3CR1)/Bcgen
Common name
B-hCX3CR1 mice
Background
C57BL/6J
Catalog number
110101
Aliases
CX3CR1, C-X3-C motif chemokine receptor 1, CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCX3CR1 mice

The exon 2 of mouse Cx3cr1 gene that encodes the full-length protein was replaced by human CX3CR1 exon 2 in B-hCX3CR1 mice.

mRNA expression analysis

Strain specific analysis of CX3CR1 gene expression in wild type (WT) mice and B-hCX3CR1 mice by RT-PCR.

Mouse Cx3cr1 mRNA was detectable only in bone marrow cells of WT mice (+/+). Human CX3CR1 mRNA was detectable only in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Monocytes

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Macrophages

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Monocytes

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Macrophages

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).