Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCX3CR1 mice
The exon 2 of mouse Cx3cr1 gene that encodes the full-length protein was replaced by human CX3CR1 exon 2 in B-hCX3CR1 mice.
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mRNA expression analysis
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Strain specific analysis of CX3CR1 gene expression in wild type (WT) mice and B-hCX3CR1 mice by RT-PCR.
Mouse Cx3cr1 mRNA was detectable only in bone marrow cells of WT mice (+/+). Human CX3CR1 mRNA was detectable only in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).
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Protein expression analysis-Monocytes
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Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).
Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.
Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).
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Protein expression analysis-Macrophages
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Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).
Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.
Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).
