Basic Information

Strain name
C57BL/6-Cx3cr1tm1(CX3CR1)/Bcgen
Common name
B-hCX3CR1 mice
Background
C57BL/6
Catalog number
110101
Aliases
CX3CR1, C-X3-C motif chemokine receptor 1, CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28

Description

CX3C chemokine receptor 1 (CX3CR1), also known as the fractalkine receptor or G-protein coupled receptor 13 (GPR13), belong to the 7-transmembrane G protein-coupled receptor (GPCR) family. CX3CR1 is mainly expressed in monocytes, macrophages, DC cells, T cells, NK cells, and microglia. CX3CR1 binds the chemokine C-X3-C motif chemokine ligand 1(CX3CL1).CX3CR1/CX3CL1 mainly mediates leukocyte adhesion and migration, and plays a role in a variety of diseases, including allergic asthma, rheumatoid arthritis, Crohn’s disease, enteritis, ischemic nephritis and atherosclerosis.CX3CR1 also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to acquired immune deficiency syndrome (AIDS). CX3CR1 variants have been described to modify the survival time and the progression rate of patients with amyotrophic lateral sclerosis. Mutations in CX3CR1 are associated to dysplasia of the hip.

Targeting strategy

Gene targeting strategy for B-hCX3CR1 mice

The exon 2 of mouse Cx3cr1 gene that encodes the full-length protein was replaced by human CX3CR1 exon 2 in B-hCX3CR1 mice.

mRNA expression analysis

Strain specific analysis of CX3CR1 gene expression in wild type (WT) mice and B-hCX3CR1 mice by RT-PCR.

Mouse Cx3cr1 mRNA was detectable only in bone marrow cells of WT mice (+/+). Human CX3CR1 mRNA was detectable only in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Monocytes

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Macrophages

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Monocytes

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis-Macrophages

Strain specific CX3CR1 expression analysis in homozygous B-hCX3CR1 mice by flow cytometry.

Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCX3CR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CX3CR1 antibody. Mouse CX3CR1 was detectable in WT mice (+/+). Human CX3CR1 was exclusively detectable in homozygous B-hCX3CR1 mice (H/H) but not in WT mice (+/+).