Basic Information

Strain Name
C57BL/6-Cxcl13tm1(CXCL13)/Cxcr5tm1(CXCR5)/Bcgen
Common Name
B-hCXCL13/hCXCR5 mice
Background
C57BL/6N
Catalog number
111189
Aliases
CXCL13:BLC, BCA1, ANGIE, BCA-1, BLR1L, ANGIE2, SCYB13 CXCR5:BLR1, CD185, MDR15
NCBI Gene ID

mRNA expression analysis

Species-specific CXCL13 and CXCR5 gene expression analysis in wild-type and humanized B-hCXCL13/hCXCR5 mice by RT-PCR. Murine Cxcl13 and Cxcr5 mRNA was detected in splenocytes isolated from wild-type C57BL/6 (+/+) mice, while human CXCL13 and CXCR5 mRNA was detected in homozygous B-hCXCL13/hCXCR5 (H/H) mice.

Protein expression analysis

Species-specific CXCL13 protein expression analysis in wild-type and humanized B-hCXCL13/hCXCR5 mice. Serum was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXCL13/hCXCR5 (H/H) mice, and analyzed by ELISA using species-specific CXCL13 kits. Murine CXCL13 protein was detected in wild-type mice, while human CXCL13 protein was detected in B-hCXCL13/hCXCR5 mice.

Species-specific CXCR5 protein expression analysis in wild-type and humanized B-hCXCL13/hCXCR5 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXCL13/hCXCR5 mice, and analyzed by flow cytometry using species-specific anti-CXCR5 antibodies. Murine CXCR5 protein was detected in wild-type mice, while human CXCR5 protein was detected in B-hCXCL13/hCXCR5 mice.

Analysis of spleen leukocytes

Analysis of spleen leukocytes in wild-type and humanized B-hCXCL13/hCXCR5 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXCL13/hCXCR5 (H/H) mice (n=3, 6-week-old), and analyzed by flow cytometry to assess leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45+ and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in homozygous B-hCXCL13/hCXCR5 mice were similar to those in wild-type mice, demonstrating that introduction of hCXCL13/hCXCR5 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these splenic cell types. Values are expressed as mean ± SEM.

Analysis of spleen T cells

Analysis of spleen T cells in wild-type and humanized B-hCXCL13/hCXCR5 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXC13/hCXCR5 (H/H) mice (female, n=3, 6-week-old), and analyzed by flow cytometry to assess leukocyte subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on CD3+ T cells and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hCXCL13/hCXCR5 mice were similar to those in wild-type mice, demonstrating that introduction of hCXCL13/hCXCR5 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these splenic T cell subtypes. Values are expressed as mean ± SEM.