Basic Information

Strain Name
C57BL/6-Cxcr2tm1(CXCR2)/Bcgen
Stock Number
110816
Common Name
B-hCXCR2 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Aliases
C-X-C motif chemokine receptor 2, CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB
Species
C57BL/6
Appearance
Black
Genotypes
Heterozygous
NCBI Gene ID

mRNA expression analysis

Species-specific CXCR2 gene expression analysis in wild-type and humanized B-hCXCR2 mice by RT-PCR. Murine Cxcr2 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CXCR2 mRNA was exclusively detected in homozygous B-hCXCR2 (H/H) mice.

Protein expression analysis in Gr-1+ cells

Protein expression analysis in Gr-1+ splenic cells

Species-specific CXCR2 protein expression analysis in humanized B-hCXCR2 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry using species-specific anti-CXCR2 antibodies. Murine CXCR2 protein was detected in wild-type mice, while human CXCR2 protein was exclusively detected in B-hCXCR2 mice.

Protein expression analysis in Gr-1+ bone marrow cells

Species-specific CXCR2 protein expression analysis in humanized B-hCXCR2 mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry using species-specific anti-CXCR2 antibodies. Murine CXCR2 protein was detected in wild-type mice, while human CXCR2 protein was exclusively detected in B-hCXCR2 mice.