Basic Information
Description
Chemokines are a group of small, mostly basic molecules that regulate cell trafficking of various leukocytes through interactions with a subset of 7-transmembrane G protein-coupled receptors. Chemokines mainly act on neutrophils, monocytes, lymphocytes, and eosinophils and play a pivotal role in host defense mechanisms. CXCR2 is a promiscuous receptor for several CXCL chemokines, including CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8. It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration.
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Details
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Strain specific analysis of CXCR2 gene expression in WT and B-hCXCR2 mice by RT-PCR. Mouse Cxcr2 mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CXCR2 mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis in Gr-1+ cells in spleen
Strain specific CXCR2 expression analysis in heterozygous B-hCXCR2 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry with species-specific CXCR2 antibody. Mouse CXCR2 was detectable in WT mice and heterozygous B-hCXCR2. Human CXCR2 was exclusively detectable in heterozygous B-hCXCR2 but not WT mice.
Protein expression analysis in Gr-1+ cells in bone marrow
Strain specific CXCR2 expression analysis in heterozygous B-hCXCR2 mice by flow cytometry. Bone marrow were collected from WT and heterozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry with species-specific CXCR2 antibody. Mouse CXCR2 was detectable in WT mice and heterozygous B-hCXCR2. Human CXCR2 was exclusively detectable in heterozygous B-hCXCR2 but not WT mice.
