B-hGLP1R mice

Basic Information

Strain Name
C57BL/6-Glp1rtm1(GLP1R)/Bcgen
Stock Number
170164
Common Name
B-hGLP1R mice
Background
C57BL/6
Aliases
GLP-1; GLP-1R; GLP-1-R

Description

The glucagon-like peptide-1 receptor (GLP1R) is a receptor protein found on beta cells of the pancreas and on neurons of the brain. It is involved in the control of blood sugar level by enhancing insulin secretion. In humans it is synthesized by the gene GLP1R, which is present on chromosome 6. It is a member of the glucagon receptor family of G protein-coupled receptors. GLP1R is composed of two domains, one extracellular (ECD) that binds the C-terminal helix of GLP-1, and one transmembrane (TMD) domain that binds the N-terminal region of GLP-1.In the TMD domain there is a fulcrum of polar residues that regulates the biased signaling of the receptor while the transmembrane helical boundaries and extracellular surface are a trigger for biased agonism.

Details

mRNA and protein expression analysis

RT-PCR and western blot analysis of GLP1R.

The mRNA of human GLP1R was detectable in the lung of the homozygous B-hGLP1R mice, but not detectable in wildtype mice.

Expression of GLP1R protein in wildtype and humanized GLP1R mice was detected by western blot. Human and mouse cross-reactive antibodies were used.

Immunohistochemical analysis

IHC for human GLP1R expression. Pancreata from wild-type (+/+) and B-hGLP1R mice (H/H) mice were stained using an antibody specific for human GLP1R. The pancreas from B-hGLP1R mice (H/H) mice showed hGLP1R positive signal.

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