Basic Information
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Targeting Strategy
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Gene targeting strategy for humanized B-hGPR35 mice.
Exon 2 of mouse Gpr35 (that encodes the full-length protein) was replaced by human GPR35 exon 2 in B-hGPR35 mice.
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mRNA Expression of GPR35
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Strain specific analysis of GPR35 gene expression in wild-type mice and B-hGPR35 mice by RT-PCR. Mouse Gpr35 mRNA was detectable in splenocytes of wild-type mice (+/+). Human GPR35 mRNA was detectable only in homozygous B-hGPR35 but not in wild-type mice.
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GPR35 Protein Expression
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Strain specific GPR35 expression analysis in homozygous humanized B-hGPR35 mice by western blot. Stomach, liver and spleen were collected from wild-type mice (+/+) and homozygous B-hGPR35 mice (H/H), and analyzed by western blot with anti-GPR35 antibody. GPR35 was detectable in wild type mice, homozygous humanized B-hGPR35 mice, and MCF-7/RAW264.7 cell line, as the antibody is crossly reactive with GPR35 in human and mice.
