Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGPRC5D mice. The exons 2-4 of mouse Gprc5d gene that encode the full-length protein were replaced by human GPRC5D exons 1-3 in B-hGPRC5D mice.
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mRNA expression analysis
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Strain specific analysis of GPRC5D gene expression in wild-type C57BL/6 mice and B-hGPRC5D mice by RT-PCR. Mouse Gprc5d mRNA was detectable only in testis of wild-type C57BL/6 mice (+/+). Human GPRC5D mRNA was detectable only in homozygous B-hGPRC5D mice (H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific GPRC5D expression analysis in homozygous B-hGPRC5D mice by western blot.
Spleen was collected from WT mice (+/+) and homozygous B-hGPRC5D (H/H) mice, and analyzed by western blot with anti-GPRC5D antibody. GPRC5D was detectable in WT mice (+/+) and homozygous B-hGPRC5D mice (H/H) due to the cross-reactivity of antibodies.
