NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hGPRC5D mice. The exons 2-4 of mouse Gprc5d gene that encode the full-length protein were replaced by human GPRC5D exons 1-3 in B-hGPRC5D mice.
Protein expression analysis
GPRC5D protein expression analysis in wild-type and humanized B-hGPRC5D mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hGPRC5D (H/H) mice, and analyzed by western blot using a cross-reactive anti-m/hGPRC5D antibody. GPRC5D protein was detected in wild-type and B-hGPRC5D mice.