Basic Information

Common Name
B-hPD-1/hPD-L1/hCCR8 mice
Catalog Number
131764
Background
C57BL/6

Protein expression analysis

Species-specific PD-1 and PD-L1 protein expression analysis in humanized B-hPD-1/hPD-L1/hCCR8 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hPD-1/hPD-L1/hCCR8 (H/H, H/H, H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry using species-specific anti-PD-1 and anti-PD-L1 antibodies. Murine PD-1 and PD-L1 proteins were detected in wild-type mice, while human PD-1 and PD-L1 proteins were exclusively detected in humanized B-hPD-1/hPD-L1/hCCR8 mice.

Species-specific CCR8 protein expression analysis in humanized B-hCCR8 mice. Murine colon cancer MC38 cells were inoculated into wild-type C57BL/6 (+/+) and homozygous B-hPD-1/hPD-L1/hCCR8 (H/H, H/H, H/H) mice. Tumors were harvested at approximately 500mm3, and analyzed by flow cytometry using species-specific anti-CCR8 antibodies. Murine CCR8 protein was detected on Tregs from wild-type mice, while human CCR8 protein was exclusively detected on Tregs from B-hPD-1/hPD-L1/hCCR8 (H/H, H/H, H/H) mice.