Basic Information
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Targeting strategy
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Gene targeting strategy for B-hα4β7 mice.
The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.
The exons 2-14 of mouse Itgb7 gene that encode the extracellular domain were replaced by human ITGB7 exons 3-15 in B-hITGB7 mice.
This mouse model was developed by breeding the B-hITGA4 mice and the B-hITGB7 mice.
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Protein expression analysis-DCs
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Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.
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Protein expression analysis-NK cells
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Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.
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Protein expression analysis-Monocytes
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Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.
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Protein expression analysis-Macrophages
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Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.
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Summary
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Protein expression analysis
Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.
