Basic Information

Strain Name
C57BL/6-Itga4tm1(ITGA4)Itgb7tm1(ITGB7)//Bcgen
Common Name
B-hα4β7 mice
Background
C57BL/6
Catalog Number
111913
Related Gene
integrin subunit alpha 4, CD49D, IA4; integrin subunit beta 7, Ly69
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hα4β7 mice. 

The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.

The exons 2-14 of mouse Itgb7 gene that encode the extracellular domain were replaced by human ITGB7 exons 3-15 in B-hITGB7 mice. 

This mouse model was developed by breeding the B-hITGA4 mice and the B-hITGB7 mice.

Protein expression analysis-DCs

Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.  

Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.

Protein expression analysis-NK cells

Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.  

Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.

Protein expression analysis-Monocytes

Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.  

Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.

Protein expression analysis-Macrophages

Strain specific ITGA4 and ITGB7 expression analysis in homozygous B-hα4β7 mice by flow cytometry.  

Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H; H/H). Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.

Summary

Protein expression analysis

Mouse ITGA4 and ITGB7 were exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice.

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