Basic Information

Strain Name
C57BL/6-Tnfrsf9tm1(TNFRSF9)Cd40tm1(CD40)/Bcgen
Stock Number
121212
Common Name
B-h4-1BB/hCD40 mice
NCBI Gene ID
Source/Investigator
4-1BB; CD137; CD137L; 4-1BB-L

Targeting strategy

Gene targeting strategy for B-h4-1BB/hCD40 mice. The exon 2-7 of mouse 4-1bb gene that encodes the extracellular domain was replaced by human 4-1BB counterpart gene in B-h4-1BB/hCD40  mice. The exon 2-7 of mouse Cd40 gene that encodes the extracellular domain was replaced by human CD40 exon 2-7 in B-h4-1BB/hCD40  mice.

Protein expression analysis

 

Strain specific CD40 and 4-1BB expression analysis in homozygous B-h4-1BB/hCD40 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-h4-1BB/hCD40 mice (H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-CD40 antibody and species-specific anti-4-1BB antibody . Mouse CD40 and 4-1BB were detectable in wild-type mice. Human CD40 and 4-1BB were exclusively detectable in homozygous B-h4-1BB/hCD40 mice (H/H) but not in wild-type mice.

Summary

Human CD40 and 4-1BB were exclusively detectable in homozygous B-h4-1BB/hCD40 mice (H/H) but not in wild-type mice.