Basic Information

Strain Name
C57BL/6-Adora2atm1(ADORA2A)/Bcgen
Stock Number
110120
Common Name
B-hA2AR mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
A2AR (ADORA2A, adenosine A2A receptor)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

A2AR encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders.

Details

mRNA expression analysis

Strain specific analysis of A2AR gene expression in WT and hA2AR mice by RT-PCR. Mouse A2ar mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human A2AR mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis in T cells

Strain specific A2AR expression analysis in homozygous B-hA2AR mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hA2AR (H/H) mice stimulated with anti-CD3ε (7.5 μg/mice, stimulation for 24 hours, i.p.) in vivo, and analyzed by flow cytometry with species-specific anti-A2AR antibody. Mouse A2AR was detectable in WT mice, human A2AR was detectable in homozygous B-hA2AR due to the anti-mouse A2AR antibody also cross-reacts with human A2AR.

Protein expression analysis in CD4+ T cells

Strain specific A2AR expression analysis in homozygous B-hA2AR mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hA2AR (H/H) mice stimulated with anti-CD3ε (7.5 μg/mice, stimulation for 24 hours, i.p.) in vivo, and analyzed by flow cytometry with species-specific anti-A2AR antibody. Mouse A2AR was detectable in WT mice, human A2AR was detectable in homozygous B-hA2AR due to the anti-mouse A2AR antibody also cross-reacts with human A2AR.

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