B-hAMHR2 mice

Basic Information

Gene targeting strategy

Gene targeting strategy for B-hAMHR2 mice. Exons 1~4 of mouse Amhr2 gene that encode the extracellular domain were replaced by human AMHR2 exons 1~4 in B-hAMHR2 mice.

mRNA expression analysis

Species-specific AMHR2 gene expression analysis in wild-type and humanized B-hAMHR2 mice by RT-PCR. Murine Amhr2 mRNA was detected in wild-type C57BL/6 (+/+) mice, while human AMHR2 mRNA was exclusively detected in homozygous B-hAMHR2 (H/H) mice.

Protein expression analysis

Species-specific AMHR2 protein expression analysis in wild-type and humanized B-hAMHR2 mice. Lung tissue was isolated from wild-type (+/+) and homozygous B-hAMHR2 (H/H) mice and analyzed by western blot using an anti-m/h AMHR2 antibody. Murine and human AMHR2 protein was detected in wild-type and B-hAMHR2 mice due to the cross-reactive antibody.

In vivo efficacy of an anti-human AMHR2 antibody

Antitumor activity of an anti-human AMHR2 antibody in humanized B-hAMHR2 mice. Humanized B-hAMHR2 ID8 cells were subcutaneously implanted into homozygous B-hAMHR2 mice (female, 6-week-old, n=6), when the tumor volume reached ~50-80mm³, mice were treated with an anti-human AMHR2 antibody (murlentamab, in house). (A) Murlentamab inhibited B-hAMHR2 ID8 tumor growth in B-hAMHR2 mice, (B) without negatively impacting body weight changes. Values are expressed as mean ± SEM.