Gene targeting strategy for B-hAMHR2 mice. The exons 1~4 of mouse Amhr2 gene that encode the extracellular domain were replaced by human AMHR2 exons 1~4 in B-hAMHR2 mice.
Protein expression analysis
Strain specific analysis of AMHR2 expression in WT and B-hAMHR2 mice by western blot. Lung were collected from WT mice and homozygous B-hAMHR2 (H/H) mice. Mouse AMHR2 was detectable in WT mice. Human AMHR2 was exclusively detectable in homozygous B-hAMHR2. The antibody is cross-reactive between human and mouse.