Basic Information
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Targeting strategy
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Gene targeting strategy for B-hAXL mice. The exons 1-11 of mouse Axl gene that encode the extracellular domain were replaced by human AXL exons 1-11 in B-hAXL mice
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Protein expression analysis
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Strain specific AXL expression analysis in homozygous B-hAXL mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAXL mice (H/H). Flow cytometry analysis of the macrophage was analyzed with species-specific anti-AXL antibody. Mouse AXL was detectable in wild-type mice. Human AXL was exclusively detectable in homozygous B-hAXL mice but not in wild-type mice.
Strain specific AXL expression analysis in homozygous B-hAXL mice by flow cytometry. Peritoneal washes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAXL mice (H/H). Flow cytometry analysis of the macrophage was analyzed with species-specific anti-AXL antibody. Mouse AXL was detectable in wild-type mice. Human AXL was exclusively detectable in homozygous B-hAXL mice but not in wild-type mice.
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Summary
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Protein expression analysis:
Human AXL was detectable in heterozygous B-hAXL mice.
