Basic Information
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Targeting strategy
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Gene targeting strategy for B-hB2M/HLA-G mice. The exons 1-3 of mouse B2m gene was replaced by human B2M and HLA-G gene in B-hB2M/HLA-G mice.
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Protein expression analysis
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Strain specific hB2M expression analysis in heterozygous B-hB2M/HLA-G (H/+) mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and heterozygous B-hB2M/HLA-G (H/+) mice, and analyzed by flow cytometry with species-specific anti-B2M antibody. Mouse B2M was detectable in wild-type mice and heterozygous B-hB2M/HLA-G (H/+). Human B2M was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) mice but not wild-type mice.
Strain specific HLA-G expression analysis in heterozygous B-hB2M/HLA-G (H/+) mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and heterozygous B-hB2M/HLA-G mice (H/+) ,and analyzed by flow cytometry with species-specific anti-HLA-G antibody. Human HLA-G was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) but not in wild-type mice.
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Summary
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B2M Protein expression:
Mouse B2m was detectable in wild-type mice and heterozygous. Human B2M was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) mice but not wild-type mice.HLA-G Protein expression:
Human HLA-G was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) but not wild-type mice.
