Basic Information

Strain name
C57BL/6-H2-T23tm1(B2M/HLA-E)Bcgen/Bcgen
Common name
B-hB2M/HLA-E mice
Background
C57BL/6
Catalog number
111352
Aliases
IMD43; QA1, HLA-6.2
NCBI gene ID
567, 3133

Targeting strategy

Gene targeting strategy for B-hB2M/HLA-E mice.

The exons 1-5 of mouse H2-T23 gene that encode extracellular domain and transmembrane domain is replaced by human HLA-E gene counterparts fused to human B2M through a flexibe (G4S)3 linker in B-hB2M/HLA-E mice. The genomic region of mouse H2-T23 gene that encodes cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric HLA-E expression is driven by endogenous mouse H2-T23 promoter, while mouse H2-T23 gene transcription and translation will be disrupted.

Protein expression analysis in Blood

Strain specific HLA-E and Qa-1(b) expression analysis in homozygous B-hB2M/HLA-E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hB2M/HLA-E mice (H/H) stimulated with mouse IFN gamma (50 μg, qdx3, i.p.) (Novoprotein, C746) in vivo. Protein expression was analyzed with anti-Qa-1(b) antibody (BD, 566640) and anti-HLA-E antibody (Biolegend, 342606) by flow cytometry. HLA-E was detectable in homozygous mice only. Qa-1(b) was detectable in C57BL/6 only.

Strain specific HLA-E and Qa-1(b) expression analysis in homozygous B-hB2M/HLA-E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hB2M/HLA-E mice (H/H) stimulated with mouse IFN gamma (50 μg, qdx3, i.p.) (Novoprotein, C746) in vivo. Protein expression was analyzed with anti-Qa-1(b) antibody (BD, 566640) and anti-HLA-E antibody (Biolegend, 342606) by flow cytometry. HLA-E was detectable in homozygous mice only. Qa-1(b) was detectable in C57BL/6 only.

Strain specific B2M expression analysis in homozygous B-hB2M/HLA-E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hB2M/HLA-E mice (H/H) stimulated with mouse IFN gamma (50 μg, qdx3, i.p.) (Novoprotein, C746) in vivo. Protein expression was analyzed with anti-mouse B2m antibody (Biolegend, 154504) and anti-human B2M antibody (Biolegend, 316311) by flow cytometry. Human B2M was detectable in homozygous mice only. Mouse B2m was detectable in homozygous mice and C57BL/6.

Strain specific B2M expression analysis in homozygous B-hB2M/HLA-E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hB2M/HLA-E mice (H/H) stimulated with mouse IFN gamma (50 μg, qdx3, i.p.) (Novoprotein, C746) in vivo. Protein expression was analyzed with anti-mouse B2m antibody (Biolegend, 154504) and anti-human B2M antibody (Biolegend, 316311) by flow cytometry. Human B2M was detectable in homozygous mice only. Mouse B2m was detectable in homozygous mice and C57BL/6.

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