Basic Information
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Targeting strategy
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Gene targeting strategy for B-hB7-H3 mice(C).
The exon 3~4 of mouse Cd276 gene that encode the extracellular domain were replaced by human CD276 exon 3~6 in B-hB7-H3 mice (C).
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Protein expression analysis
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Species-specific B7-H3 protein expression analysis in homozygous B-hB7-H3 mice (C). Tissues were collected from wild-type BALB/c (+/+) and homozygous B-hB7-H3 (C) (H/H) mice, and analyzed by western blot using an anti-human B7-H3 antibody. Human B7-H3 protein was detected in B-hB7-H3 mice (C).
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mRNA expression analysis
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Strain specific analysis of B7-H3 mRNA expression in wild-type BALB/c mice and B-hB7-H3 mice (C) by RT-PCR. Spleen RNA was isolated from wild-type BALB/c mice (+/+) and homozygous B-hB7-H3 mice (C) (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human B7-H3 primers. Mouse B7h3 mRNA was detectable only in wild-type BALB/c mice. Human B7-H3 mRNA was detectable only in homozygous B-hB7-H3 mice (C) but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse B7h3 mRNA was detectable in spleen of wild-type BALB/c mice. Human B7-H3 mRNA was detectable in B-hB7-H3 mice (C).
Protein expression analysis:
Human B7-H3 was detectable in homozygous B-hB7-H3 mice (C) but not in wild-type BALB/c mice.
