Basic Information

Strain name
C57BL/6-Vtcn1tm1(VTCN1)/Bcgen
Common name
B-hB7-H4 mice
Background
C57BL/6
Catalog number
110045
Aliases
B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1
NCBI Gene ID

mRNA expression analysis

Strain specific analysis of B7-H4 gene expression in wild type and B-hB7-H4 mice by RT-PCR.

Mouse B7-h4 mRNA was detectable only in ovary of wild type mice (+/+). Human B7-H4 mRNA was detectable only in homozygous B-hB7-H4 mice (H/H) but not in wild type mice.

Protein expression analysis

Strain specific B7-H4 expression analysis in homozygous B-hB7-H4 mice by western blot.

Testis tissue was collected from wild type mice (+/+) and homozygous B-hB7-H4 mice (H/H) and analyzed by western blot with anti-B7-H4 antibody. Mouse B7-H4 was detectable in wild type mice. Human B7-H4 was detectable in homozygous B-hB7-H4 but not in wild type mice. Anti-B7-H4 antibody is crossly reactive with B7-H4 in human and mice.