Basic Information

Strain name
C57BL/6-Vtcn1tm1(VTCN1)/Bcgen
Common name
B-hB7-H4 mice
Background
C57BL/6J
Catalog number
110045
Aliases
B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1
NCBI Gene ID

mRNA Expression Analysis

Species-specific B7-H4 gene expression analysis in wild type and B-hB7-H4 mice by RT-PCR. Murine B7-h4 mRNA was detected in ovaries isolated from wild-type (+/+) mice, while human B7-H4 mRNA was detected in homozygous B-hB7-H4 (H/H) mice.

Protein Expression Analysis

B7-H4 protein expression analysis in homozygous B-hB7-H4 mice by western blot. Testis tissue was collected from wild type (+/+) mice and homozygous B-hB7-H4 (H/H) mice and analyzed by western blot using an anti-B7-H4 antibody. Mouse B7-H4 protein was detected in wild type mice, while human B7-H4 protein was detected in homozygous B-hB7-H4 mice. Anti-B7-H4 antibody is cross-reactive with B7-H4 in human and mice.

In vivo Efficacy of Anti-Human B7-H4 Antibody

Antitumor activity of an anti-human B7-H4 antibody in humanized B-hB7-H4 mice. Humanized murine colon cancer B-hB7-H4 MC38 cells (3ⅹ106) were subcutaneously implanted into homozygous B-hB7-H4 mice (female, 8-week-old, n=6). B-hB7-H4 mice were grouped when tumor volume reached approximately 100 mm3, at which time FPA-150 (anti-human B7-H4 Ab, in house) was administered at doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown, FPA-150 inhibited tumor growth without negatively impacting body weight changes, demonstrating that B-hB7-H4 mice provide a powerful preclinical model for in vivo evaluation of anti-human B7-H4 antibodies. Values are expressed as mean ± SEM.

Poster

AACR 2022: A Humanized Mouse Model of the Promising Immune Checkpoint Molecule B7-H4