Basic Information

Strain Name
C57BL/6-Ccr4tm1(CCR4)/Bcgen
Stock Number
110098
Common Name
B-hCCR4 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CCR4 (C-C motif chemokine receptor 4), CD194

Description

C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene. CCR4 has also recently been designated CD194 (cluster of differentiation 194). CCR4 belongs to the G protein-coupled receptor family. It is a receptor for the following CC chemokines: CCL2, CCL4, CCL5, CCL17, CCL22. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. CCR4 is often expressed on leukemic cells in cutaneous T-cell lymphoma (CTCL). Mogamulizumab is a humanized monoclonal antibody targeted at CCR4 and is an investigational drug for CTCL.

Details

Protein expression analysis in CD4 T cells

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

Protein expression analysis in Tregs

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

Protein expression analysis in Tregs

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

B-CAG-hCCR4 MC38 bind anti-human CCR4 antibody

Analysis of B-CAG-hCCR4 MC38 by FACS. Flow cytometry analysis of the B-CAG-hCCR4 MC38 was performed to assess anti-human CCR4 Ab binding. Single live cells were gated for CD45- population and used for further analysis as indicated here. B-CAG-hCCR4 MC38 can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.

T cells in B-hCCR4 mice bind anti-human CCR4 antibody

Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. T cells of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.

Tregs in B-hCCR4 mice bind anti-human CCR4 antibody

Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. Tregs of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.

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