Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD155/hCD112 mice.
The exons 2-6 of mouse Cd155 gene that encode the extracellular region were replaced by human CD155 exons 2-6 in B-hCD155/hCD112 mice.
The exons 2-6 of mouse Cd112 gene that encode the extracellular region were replaced by human CD112 exons 2-6 in B-hCD155/hCD112 mice.
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mRNA expression analysis
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Strain specific analysis of CD112 gene expression in wild-type C57BL/6 mice and B-hCD155/hCD112 mice by RT-PCR. Mouse Cd112 mRNA was detectable only in colon of wild-type C57BL/6 mice (+/+). Human CD112 mRNA was detectable only in homozygous B-hCD155/hCD112 mice (H/H;H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific CD155 expression analysis in heterozygous B-hCD155/hCD112 mice by flow cytometry. Dendritic cells of spleen and macrophages of peritoneal lavage fluid were collected from wild-type C57BL/6 mice (+/+;+/+) and heterozygous B-hCD155/hCD112 mice (H/+;H/+), and analyzed by flow cytometry with species-specific anti-CD155 antibody. Mouse CD155 was detectable in wild-type mice and heterozygous B-hCD155/hCD112 mice. Human CD155 was exclusively detectable in heterozygous B-hCD155/hCD112 mice but not in wild-type mice.
Strain specific CD112 expression analysis in heterozygous B-hCD155/hCD112 mice by flow cytometry. Dendritic cells of spleen and macrophages of peritoneal lavage fluid were collected from wild-type C57BL/6 mice (+/+;+/+) and heterozygous B-hCD155/hCD112 mice (H/+;H/+), and analyzed by flow cytometry with species-specific anti-CD112 antibody. Mouse CD112 was detectable in wild-type mice and heterozygous B-hCD155/hCD112 mice. Human CD112 was exclusively detectable in heterozygous B-hCD155/hCD112 mice but not in wild-type mice.
Strain specific CD155 expression analysis in homozygous B-hCD155/hCD112 mice by flow cytometry. Dendritic cells, macrophages and monocytes of spleen were collected from wild-type C57BL/6 mice (+/+;+/+) and homozygous B-hCD155/hCD112 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD155 antibody. Mouse CD155 was detectable in wild-type mice. Human CD155 was exclusively detectable in homozygous B-hCD155/hCD112 mice but not in wild-type mice.
Strain specific CD112 expression analysis in homozygous B-hCD155/hCD112 mice by flow cytometry. Dendritic cells, macrophages and monocytes of spleen were collected from wild-type C57BL/6 mice (+/+;+/+) and homozygous B-hCD155/hCD112 mice (H/H;H/H), and analyzed by flow cytometry with anti-CD112 antibodies. Mouse CD112 was detectable in wild-type mice. Human CD112 was exclusively detectable in heterozygous B-hCD155/hCD112 mice but not in wild-type mice. Anti-mCD112 antibody is a human-mouse cross-binding antibody.
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Summary
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- mRNA expression analysis:
Mouse Cd112 mRNA was detectable only in colon of wild-type C57BL/6 mice (+/+). Human CD112 mRNA was detectable only in homozygous B-hCD155/hCD112 mice (H/H;H/H), but not in wild-type mice.
- Protein expression analysis:
Mouse CD155 and CD112 were detectable on dendritic cells of spleen and macrophages of peritoneal lavage fluid in wild-type mice and heterozygous B-hCD155/hCD112 mice. Human CD155 and CD112 were exclusively detectable in heterozygous B-hCD155/hCD112 mice but not in wild-type mice.
Mouse CD155 and CD112 was detectable on dendritic cells, macrophages and monocytes of spleen in wild-type mice. Human CD155 and CD112 was exclusively detectable in homozygous B-hCD155/hCD112 mice but not in wild-type mice.
