Basic Information

Strain Name
C57BL/6-Cd180tm2(CD180)/Bcgen
Common Name
B-hCD180 mice
Background
C57BL/6N
Catalog number
111221
Related Genes
LY64, Ly78, RP105
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD180 mice.

The exons 2-3 of mouse Cd180 gene were replaced by human CD180 exons 2-3 in B-hCD180 mice.

Protein expression analysis

Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. 

Splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD180 mice (H/H) ,and analyzed by flow cytometry with species-specific anti-CD180 antibody. Mouse CD180 was detectable in wild-type mice. Human CD180 was exclusively detectable in homozygous B-hCD180 but not in wild-type mice.

Summary

Protein expression:

Mouse CD180 was detectable in wild-type mice. Human CD180 was exclusively detectable in homozygous B-hCD180 but not in wild-type mice.

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