Basic Information
Strain Name
C57BL/6-Cd180tm2(CD180)/Bcgen
Common Name
B-hCD180 mice
Background
C57BL/6N
Catalog number
111221
Related Genes
LY64, Ly78, RP105
NCBI Gene ID
-
Targeting strategy
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Gene targeting strategy for B-hCD180 mice.
The exons 2-3 of mouse Cd180 gene were replaced by human CD180 exons 2-3 in B-hCD180 mice.
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Protein expression analysis
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Species-specific CD180 protein expression analysis in humanized B-hCD180 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD180 (H/+) mice and analyzed by flow cytometry using species-specific anti-CD180 antibodies. Murine CD180 protein was detected in wild-type and B-hCD180 mice, while human CD180 protein was exclusively detected in B-hCD180 mice.
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Summary
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Protein expression：
Mouse CD180 was detectable in wild-type mice. Human CD180 was exclusively detectable in homozygous B-hCD180 but not in wild-type mice.
