LY64, Ly78, RP105
NCBI Gene ID
Gene targeting strategy for B-hCD180 mice.
The exons 2-3 of mouse Cd180 gene were replaced by human CD180 exons 2-3 in B-hCD180 mice.
Protein expression analysis
Species-specific CD180 protein expression analysis in humanized B-hCD180 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD180 (H/+) mice and analyzed by flow cytometry using species-specific anti-CD180 antibodies. Murine CD180 protein was detected in wild-type and B-hCD180 mice, while human CD180 protein was exclusively detected in B-hCD180 mice.
Mouse CD180 was detectable in wild-type mice. Human CD180 was exclusively detectable in homozygous B-hCD180 but not in wild-type mice.