Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD19 mice.
The coding sequence of human extracellular domain, mouse transmembrane and cytoplasmic domain was inserted into murine exon 2 in B-hCD19 mice.
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Protein expression analysis
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Strain specific CD19 expression analysis in heterozygous B-hCD19 mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hCD19 mice (H/+), and analyzed by flow cytometry with anti-CD19 antibody. Mouse CD19 was detectable in wild-type mice and heterozygous B-hCD19 mice (H/+). Human CD19 was exclusively detectable in heterozygous B-hCD19 mice (H/+).
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Summary
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Protein expression analysis:
Human CD19 was exclusively detectable on B cells in blood of heterozygous B-hCD19 mice, and mouse CD19 was detectable in wild-type C57BL/6 mice and heterozygous B-hCD19 mice.
