Basic Information

Strain Name
C57BL/6JNifdc-Cd19tm4(CD19)/Bcgen
Common Name
B-hCD19 mice
Background
C57BL/6JNifdc
Catalog number
112657
Aliases
B4, CVID3
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD19 mice.

The coding sequence of human extracellular domain, mouse transmembrane and cytoplasmic domain was inserted into murine exon 2 in B-hCD19 mice.

Protein expression analysis

Strain specific CD19 expression analysis in heterozygous B-hCD19 mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hCD19 mice (H/+), and analyzed by flow cytometry with anti-CD19 antibody. Mouse CD19 was detectable in wild-type mice and heterozygous B-hCD19 mice (H/+). Human CD19 was exclusively detectable in heterozygous B-hCD19 mice (H/+).

Summary

Protein expression analysis:

Human CD19 was exclusively detectable on B cells in blood of heterozygous B-hCD19 mice, and mouse CD19 was detectable in wild-type C57BL/6 mice and heterozygous B-hCD19 mice.

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