NCBI Gene ID
CD20, MS4A1,, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1
Gene targeting strategy
The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD20 mice.
mRNA expression analysis
Species-specific CD20 gene expression analysis in wild-type and humanized B-hCD20 mice by RT-PCR. Murine Cd20 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD20 mRNA was detected in homozygous B-hCD20 (H/H) mice.
Protein expression analysis
Species-specific CD20 protein expression analysis in wild-type and humanized B-hCD20 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD20 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD20 antibodies. Murine CD20 protein was detected in wild-type mice, while human CD20 protein was detected in B-hCD20 mice.