Basic Information
Strain name
C57BL/6–Ms4a1tm2(MS4A1)/Bcgen
Common name
B-hCD20 mice
Catalog number
111231
Background
C57BL/6N
NCBI Gene ID
Aliases
CD20, MS4A1,, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1
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Gene targeting strategy
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The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD20 mice.
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mRNA expression analysis
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Species-specific CD20 gene expression analysis in wild-type and humanized B-hCD20 mice by RT-PCR. Murine Cd20 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD20 mRNA was detected in homozygous B-hCD20 (H/H) mice.
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Protein expression analysis
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Species-specific CD20 protein expression analysis in wild-type and humanized B-hCD20 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD20 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD20 antibodies. Murine CD20 protein was detected in wild-type mice, while human CD20 protein was detected in B-hCD20 mice.
