Basic Information

Strain name
C57BL/6Ms4a1tm2(MS4A1)/Bcgen
Common name
B-hCD20 mice
Catalog number
111231
Background
C57BL/6
NCBI Gene ID
Aliases
CD20, MS4A1,, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1

Targeting strategy

The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD20 mice.

mRNA expression analysis

Strain specific analysis of CD20 gene expression in wild type (WT) mice and B-hCD20 mice by RT-PCR.

Mouse CD20 mRNA was detectable only in splenocytes of WT mice (+/+). Human CD20 mRNA was detectable only in homozygous B-hCD20 mice (H/H) but not in WT mice (+/+).

Protein expression analysis in B cells

Strain specific CD20 expression analysis in homozygous B-hCD20 mice by flow cytometry.

Splenocytes were collected from WT and homozygous B-hCD20 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice. Human CD20 was exclusively detectable in homozygous B-hCD20 but not WT mice.