Basic Information

Strain name
C57BL/6-Cd200tm1(CD200)/Bcgen
Common name
B-hCD200 mice
Background
C57BL/6
Catalog number
111133
Aliases
CD200, MOX1, MOX2, MRC, OX-2, CD200 molecule
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD200 mice.

The exons 1-5 of mouse Cd200 gene that encode the full-length protein were replaced by human CD200 exons 1-7 in B-hCD200 mice.

Protein expression analysis

Strain specific CD200 expression analysis in heterozygous B-hCD200 mice by flow cytometry. 

Splenocytes were collected from wild type (WT) mice (+/+) and heterozygous B-hCD200 mice (H/+), and analyzed by flow cytometry with species-specific anti-CD200 antibody. Mouse CD200 was detectable in WT mice (+/+) and heterozygous B-hCD200 mice (H/+). Human CD200 was exclusively detectable in heterozygous B-hCD200 mice (H/+) but not in WT mice (+/+).

Summary

Protein expression analysis:

Human CD200 was exclusively detectable on B cells of heterozygous B-hCD200 mice (H/+) but not in WT mice (+/+), and mouse CD200 was detectable in WT mice (+/+) and heterozygous B-hCD200 mice (H/+) .