Gene targeting strategy for B-hCD200R mice.
The exons 2-5 of mouse Cd200r1 gene that encode the extracellular domain were replaced by human CD200R1 exons 2-5 in B-hCD200R mice.
Protein expression analysis
Species-specific CD200R protein expression analysis in humanized B-hCD200R mice. Peritoneal exudate macrophages were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD200R (H/+) mice, and analyzed by flow cytometry using species-specific anti-CD200R antibodies. Murine CD200R protein was detected in wild-type and heterozygous B-hCD200R (H/+) mice, while human CD200R protein was exclusively detected in B-hCD200R mice.