Basic Information

Strain Name
C57BL/6-Cd22tm1(CD22)/Bcgen
Stock Number
110775
Common Name
B-hCD22 mice
Background
C57BL/6
Related Genes
cluster of differentiation-22, SIGLEC2
Targeting Strategy
The exons 4~11 of mouse Cd22 gene that encode the extracellular domain were replaced by human CD22 exons 4~11 in B-hCD22 mice.
NCBI Gene ID

Details

protein expression analysis

Strain specific CD22 expression analysis in heterozygous B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD22 (H/+) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice and heterozygous B-hCD22. Human CD22 was exclusively detectable in heterozygous B-hCD22 but not WT mice.

 

Strain specific CD22 expression analysis in heterozygous B-hCD22 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD22 (H/+) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice and heterozygous B-hCD22. Human CD22 was exclusively detectable in heterozygous B-hCD22 but not WT mice.

Strain specific CD22 expression analysis in homozygouse B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD22 (H/H) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice. Human CD22 was exclusively detectable in homozygous B-hCD22 but not WT mice.

 

mRNA expression analysis

Strain specific analysis of CD22 gene expression in WT and homozygous B-hCD22 mice by RT-PCR. Mouse Cd22 mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CD22 mRNA was detectable only in H/H, but not in +/+ mice.

protein expression analysis

Strain specific CD22 expression analysis in homozygouse B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD22 (H/H) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice. Human CD22 was exclusively detectable in homozygous B-hCD22 but not WT mice.