Basic Information
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mRNA Expression Analysis
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Species-specific CD22 gene expression analysis in wild-type and humanized B-hCD22 mice. Murine Cd22 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD22 mRNA was exclusively detected in B-hCD22 mice.
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Protein Expression Analysis
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Species-specific CD22 protein expression analysis in humanized B-hCD22 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD22 (H/+) mice and analyzed by flow cytometry using species-specific CD22 antibodies. Murine CD22 protein was detected in wild-type and heterozygous B-hCD22 mice, while human CD22 protein was exclusively detected in B-hCD22 mice.
Species-specific CD22 protein expression analysis in humanized B-hCD22 mice. Blood was collected from wild-type C57BL/6 (+/+) and heterozygous B-hCD22 (H/+) mice and analyzed by flow cytometry using species-specific CD22 antibodies. Murine CD22 protein was detected in wild-type and heterozygous B-hCD22 mice, while human CD22 protein was exclusively detected in B-hCD22 mice.
Species-specific CD22 protein expression analysis in humanized B-hCD22 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD22 (H/H) mice and analyzed by flow cytometry using species-specific CD22 antibodies. Murine CD22 protein was detected in wild-type mice, while human CD22 protein was exclusively detected in B-hCD22 mice.