Basic Information
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Protein expression analysis in blood-Red blood cells
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Red blood cells were collected from wild-type mice (+/+), homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was detectable in wild-type mice, but not detectable in B-hCD24 mice plus. Human CD24 was not detectable in homozygous B-hCD24 mice plus.
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Protein expression analysis in blood and spleen-B cells
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Blood cells and splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was detectable in wild-type mice blood(A) and spleen(B). Human CD24 was detectable in homozygous B-hCD24 mice plus blood(A) and spleen(B).
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Protein expression analysis in blood and spleen-T cells
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Blood cells and splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was weakly detectable in wild-type mice blood(A) while mouse CD24 can not be detected in wild-type mice spleen(B). Human CD24 was not detectable in homozygous B-hCD24 mice plus blood(A) and spleen(B).
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Protein expression analysis in blood and spleen-NK cells
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Blood cells and splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was not detectable in wild-type mice blood(A) and spleen(B). Human CD24 was not detectable in homozygous B-hCD24 mice plus blood(A) and spleen(B).
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Protein expression analysis in blood and spleen-Granulocytes
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Blood cells and splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was detectable in wild-type mice blood(A) and spleen(B). Human CD24 was detectable in homozygous B-hCD24 mice plus blood(A) and spleen(B).
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Protein expression analysis in blood and spleen-DC cells
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Strain specific CD24 expression analysis in homozygous B-hCD24 mice plus by flow cytometry. Blood cells and splenocytes were collected from wild-type mice (+/+) and homozygous B-hCD24 mice plus analyzed by flow cytometry with species-specific anti-CD24 antibody. Mouse CD24 was detectable in wild-type mice blood(A) and spleen(B). Human CD24 was detectable in homozygous B-hCD24 mice plus blood(A) and spleen(B).
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Antibody binding assay-the Ab1
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Analysis of blood and splenocytes of B-hCD24 mice plus by FACS. Blood cells and splenocytes were collected from homozygous B-hCD24 mice plus. Flow cytometry analysis of the blood and splenocytes were performed to assess anti-human CD24 antibody Ab1 binding with B cell and macrophages. B cell and macrophages in homozygous B-hCD24 mice plus bind well with Ab1 vs isotype control. (All antibodies were provided by client)
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Summary
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Protein expression analysis:
Human CD24 was exclusively detected in B cells, granulocytes, macrophages and DC cells. Furthermore, human CD24 was not detectable in erythrocyte. We predicate that CD24 has similar expression pattern between human and B-hCD24 mice plus.
Antibody binding assay:
Blood cells and splenocytes were collected from homozygous B-hCD24 mice plus. Flow cytometry analysis of the blood and splenocytes were performed to assess anti-human CD24 antibody Ab1 and Ab2 binding with B cell and macrophages. B cell and macrophages in homozygous B-hCD24 mice plus bind well with Ab1 and Ab2 vs isotype control.
