Basic Information

Strain Name
C57BL/6-Cd24atm1(CD24)/Bcgen
Stock Number
110767
Common Name
B-hCD24 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD24, CD24A, PRO940
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

Signal transducer CD24 also known as cluster of differentiation 24 or heat stable antigen CD24 (HSA) is a protein that in humans is encoded by the CD24 gene. CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. The protein also contributes to a wide range of downstream signaling networks and is crucial for neural development. Cross-linking of CD24 on the surface of neutrophils induces apoptosis, and this appears to be defective in sepsis. Genetic ablation and therapeutic blockade of CD24 resulted in a macrophage-dependent reduction of tumor growth in vivo and an increase in survival time. Tumor-expressed CD24 in promoting immune evasion through its interaction with the inhibitory receptor sialic acid-binding Ig-like lectin-10, which is expressed by tumor-associated macrophages.

Details

mRNA expression analysis in T cells

Strain specific analysis of CD24 gene expression in WT and hCD24 mice by RT-PCR. Mouse Cd24 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CD24 mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis in T cells

Strain specific CD24 expression analysis in homozygous B-hCD24 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD24 (H/H) mice, and analyzed by flow cytometry with species-specific CD24 antibody. Mouse CD24 was detectable in WT mice. Human CD24 was exclusively detectable in homozygous B-hCD24 but not WT mice.

Protein expression analysis in B cells

Strain specific CD24 expression analysis in homozygous B-hCD24 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD24 (H/H) mice, and analyzed by flow cytometry with species-specific CD24 antibody. Mouse CD24 was detectable in WT mice. Human CD24 was exclusively detectable in homozygous B-hCD24 but not WT mice.

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