Basic Information

Strain name
C57BL/6-Cd28tm1(CD28)Ms4a1tm2(MS4A1)/Bcgen
Common name
B-hCD28/hCD20 mice
Background
C57BL/6
Catalog number
121253
NCBI Gene ID
Related genes
CD28, CD28 molecule, Tp44; CD20, MS4A1, membrane spanning 4-domains A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7

Targeting strategy

Gene targeting strategy for B-hCD28/hCD20 mice. The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hCD20 mice. The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD28/hCD20 mice.

Protein expression analysis

Strain specific CD28 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice. Human CD28 was exclusively detectable in homozygous B-hCD28/hCD20 but not WT mice.

Strain specific CD20 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice. Human CD20 was exclusively detectable in homozygous B-hCD28/hCD20 but not WT mice.

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