Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD28/hCD20 mice. The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hCD20 mice. The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD28/hCD20 mice.
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Protein expression analysis
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Strain specific CD28 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice. Human CD28 was exclusively detectable in homozygous B-hCD28/hCD20 but not WT mice.
Strain specific CD20 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice. Human CD20 was exclusively detectable in homozygous B-hCD28/hCD20 but not WT mice.
