Basic Information

Strain Name
C57BL/6-Fcgr2btm1(FCGR2B)/Bcgen
Common Name
B-hCD32B mice
Background
C57BL/6J
Catalog number
112052
Aliases
CD32, CD32B, FCGR2, FCG2.
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hFCGR2B mice. Exons 2~3 of the mouse Fcg2b gene that encodes the extracellular domain were replaced by human FCGR2B exons 3-5 in B-hCD32B mice.

Protein expression analysis in blood cells

Species-specific CD32B protein expression analyses in wild-type and humanized B-hCD32B mice. Blood-derived dendritic cells and monocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD32B (H/+) mice and analyzed by flow cytometry using species-specific anti-CD32B antibodies. (A) Mouse CD32B protein was detected in DCs from wild-type and heterozygous B-hCD32B mice, while human CD32B protein was only detected in heterozygous B-hCD32B mice. (B) Mouse CD32B protein was detected in monocytes from wild-type and heterozygous B-hCD32B mice, while human CD32B protein was only detected in B-hCD32B mice.

Protein expression analysis in lymphocytes

Species-specific CD32B protein expression analysis in wild-type and humanized B-hCD32B mice. Lymphocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD32B (H/+) mice and analyzed by flow cytometry using species-specific anti-CD32B antibodies. (A) Mouse CD32B protein was detected in dendritic cells from wild-type and heterozygous B-hCD32B mice, while human CD32B protein was only detected in B-hCD32B mice. (B) Mouse CD32B protein was detected in monocytes from wild-type and heterozygous B-hCD32B mice, while human CD32B protein was only detected in B-hCD32B mice.