Basic Information

Strain Name
C57BL/6-Cd33tm1(CD33)/Bcgen
Stock Number
110823
Common Name
B-hCD33 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
SIGLEC-3, SIGLEC3, p67
Species
C57BL/6J
Appearance
Black
Genotypes
Heterozygous
NCBI Gene ID

Details

mRNA expression analysis

Strain specific analysis of CD33 gene expression in WT and heterozygous hCD33 mice by RT-PCR. Mouse Cd33 mRNA was detectable in lung of WT and heterozygous B-hCD33. Human CD33 mRNA was detectable only in H/+, but not in +/+ mice.

Protein expression analysis in CD11b+ cells in blood

Strain specific CD33 expression analysis in heterozygous B-hCD33 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD33 mice, and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in WT and heterozygous B-hCD33. Human CD33 was exclusively detectable in heterozygous B-hCD33 but not WT mice.

Protein expression analysis in CD11b+ cells in spleen

Strain specific CD33 expression analysis in heterozygous B-hCD33 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD33 mice, and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in WT and heterozygous B-hCD33. Human CD33 was exclusively detectable in heterozygous B-hCD33 but not WT mice.