Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hCD33 mice. The exons 1~3 of mouse Cd33 gene that the encode extracellular domain were replaced by human CD33 exons 1~3 in B-hCD33 mice.
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mRNA Expression Analysis
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Strain specific analysis of CD33 gene expression in wild-type mice and B-hCD33 mice by RT-PCR. Mouse CD33 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CD33 mRNA was detectable only in homozygous B-hCD33 but not in wild-type mice.
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Protein Expression Analysis in Macrophages in Bone Marrow
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Strain specific CD33 expression analysis in homozygous B-hCD33 mice by flow cytometry. Bone marrow cells were collected from wild-type mice (+/+) and homozygous B-hCD33 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in wild-type mice. Human CD33 was exclusively detectable in homozygous B-hCD33 but not in wild-type mice.
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Protein Expression Analysis in Gr-1+ Cells in Bone Marrow
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Strain specific CD33 expression analysis in homozygous B-hCD33 mice by flow cytometry. Bone marrow cells were collected from wild-type mice (+/+) and homozygous B-hCD33 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in wild-type mice. Human CD33 was exclusively detectable in homozygous B-hCD33 but not in wild-type mice.
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hCD33 Expression Analysis in Brain Of B-hCD33 Mice
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Strain specific CD33 expression analysis in homozygous B-hCD33 mice by multiplex immunofluorescence staining. Brain tissue was collected from wild type C57BL/6 mice and homozygous B-hCD33 mice (female, 7-week-old) and processed into formalin-fixed paraffin-embedded tissue sections. The paraffin sections were immunolabeling using species-specific anti-human CD33 antibody (red) and the microglial marker IBA1 antibody (green). The results indicated that human CD33 is expressed in brain of B-hCD33 mice.
