Basic Information
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Details
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mRNA expression analysis
Strain specific analysis of CD33 gene expression in WT and heterozygous hCD33 mice by RT-PCR. Mouse Cd33 mRNA was detectable in lung of WT and heterozygous B-hCD33. Human CD33 mRNA was detectable only in H/+, but not in +/+ mice.
Protein expression analysis in CD11b+ cells in blood
Strain specific CD33 expression analysis in heterozygous B-hCD33 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD33 mice, and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in WT and heterozygous B-hCD33. Human CD33 was exclusively detectable in heterozygous B-hCD33 but not WT mice.
Protein expression analysis in CD11b+ cells in spleen
Strain specific CD33 expression analysis in heterozygous B-hCD33 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD33 mice, and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in WT and heterozygous B-hCD33. Human CD33 was exclusively detectable in heterozygous B-hCD33 but not WT mice.
