NCBI Gene ID
Gene Targeting Strategy
Exons 2-6 of the murine Cd3e gene, which encodes the extracellular domain was replaced by human CD3E exons 2-7 in B-hCD3E/hIL2RA mice. Exons 2-6 of the murine Il2ra gene, which encodes the extracellular domain was replaced by human IL2RA exons 2-6 in B-hCD3E/hIL2RA mice.
Protein Expression Analysis
Species-specific CD3E and IL2RA expression analysis in double humanized B-hCD3E/hIL2RA mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD3E/hIL2RA (H/H) mice and analyzed by flow cytometry using species-specific anti-CD3E and anti-IL2RA antibodies. Human CD3E and human IL2RA protein was exclusively detected in B-hCD3E/hIL2RA mice compared to wild-type mice.