Basic Information

Strain name
C57BL/6-Ptprctm1(PTPRC)/Bcgen
Common name
B-hCD45 mice
Catalog number
110973
Background
C57BL/6
Aliases
LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD45 mice. The extracellular region of mouse Cd45 gene was replaced by the counterpart gene sequences of human CD45 in B-hCD45 mice.

Protein expression analysis

Strain specific CD45 expression analysis in heterozygous B-hCD45 mice by flow cytometry. Blood was collected from wild-type C57BL/6 (+/+) and heterozygous B-hCD45 mice (H/+) and analyzed by flow cytometry with species-specific anti-CD45 antibody. Mouse CD45 was detectable in wild-type mice and heterozygous B-hCD45 mice. Human CD45 was exclusively detectable in heterozygous B-hCD45 mice but not in wild-type mice.