CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation and immunity. CD47 is an important “self” mark on the cell surface and inhibits macrophagocytosis by interacting with SIRPα on macrophage surface. Animal studies have shown that CD47 antibody is an effective treatment for multiple types of tumors. Therefore, CD47 is a promising new target for tumor immunotherapy following PD- 1/PD-L1 axis.
mRNA Expression Analysis
RT-PCR analysis of CD47 gene. The hCD47 mRNA, but not mCD47 mRNA was detected in homozygous B-hCD47 (C) mice.
Protein Expression Analysis
Splenocytes from both wild type (WT)BALB/c and homozygous B-hCD47 (C) mice were analyzed by flow cytometry. Mouse CD47+ cells were detectable in WT BALB/c, while human CD47+ cells were only detectable in the homozygous B-hCD47 (C) mice.
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