Gene targeting strategy for B-hCD5 mice. The exons 2~7 of mouse Cd5 gene that encode the extracellular domain were replaced by human CD5 exons 2~7 in B-hCD5 mice.
mRNA expression analysis
Strain specific analysis of CD5 gene expression in WT and homozygous B-hCD5 mice by RT-PCR. Mouse Cd5 mRNA was detectable in thymocytes of wild-type (+/+) mice. Human CD5 mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific CD5 expression analysis in homozygous B-hCD5 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD5 (H/H) mice, and analyzed by flow cytometry with species-specific CD5 antibody. Mouse CD5 was detectable in WT mice. Human CD5 was exclusively detectable in homozygous B-hCD5 but not WT mice.