Basic Information

Strain Name
C57BL/6-Cd5tm1(CD5)/Bcgen
Stock Number
111125
Common Name
B-hCD5 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD5, T1, LEU1
Species
C57BL/6
Appearance
Black
Genotypes
Heterozygous

Description

CD5 is a cluster of differentiation, also known as T1 and Leu-1, which is expressed on the surface of thymocytes, T cells (various species) and in a subset of murine B cells known as B-1a. CD5 is a member of the SRCR family, the extracellular region of CD5 is composed of 3 type B SRCR domains (D1, D2, and D3) each one containing 8 cysteines and encoded by a single exon (in contrast to type A, which contain 6 cysteines and are encoded by two or more exons). The nature of the endogenous CD5 ligand/s (CD5L) is reported as several counter-receptors, including CD72, gp35–40, gp150, gp200, the framework region of IgVH. CD5 is initially identified as a positive modulator of TCR signaling, but the most accepted view of CD5 is that of a negative regulator of T cell receptor signaling. CD5 also plays important roles in thymocyte, mature T cell, B cell survival, Th differentiation, suggesting that CD5 mainly acts as a pro-survival molecule in B and T cells, but under some experimental or disease conditions (such as B-CLL) it might work as a proapoptotic molecule. As CD5 modulates TCR and BCR signaling, Treg generation, anergy induction, and activation induced cell death, it can be considered an immune checkpoint inhibitor that, similarly to other negative co-stimulators such as PD-1, could be used to potentiate antitumoral T cell responses or promote immunity against infections and modulate autoimmune reactions.

Targeting strategy

Gene targeting strategy for B-hCD5 mice. The exons 2~7 of mouse Cd5 gene that encode the extracellular domain were replaced by human CD5 exons 2~7 in B-hCD5 mice.

Details

Protein expression analysis in T cells

Strain specific CD5 expression analysis in heterozygous B-hCD5 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD5 (H/+) mice, and analyzed by flow cytometry with species-specific CD5 antibody. Mouse CD5 was detectable in WT mice and heterozygous B-hCD5. Human CD5 was exclusively detectable in heterozygous B-hCD5 but not WT mice.

Protein expression analysis in B cells

Strain specific CD5 expression analysis in heterozygous B-hCD5 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD5 (H/+) mice, and analyzed by flow cytometry with species-specific CD5 antibody. Mouse CD5 was detectable in WT mice and heterozygous B-hCD5. Human CD5 was exclusively detectable in heterozygous B-hCD5 but not WT mice.

 

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