AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hCD69 mice. Exons 2-5 of mouse Cd69 gene that encode extracellular region coding sequences were replaced by human CD69 exons 2-5 in B-hCD69 mice.
Protein expression analysis
Species-specific CD69 protein expression analysis in wild-type and humanized B-hCD69 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD69 (H/H) mice, stimulated with anti-CD3ε and anti-CD28 in vitro, and analyzed by flow cytometry using species-specific anti-CD69 antibodies. Mouse CD69 protein was detected on T cells, B cells and NK cells in wild-type mice. Human CD69 protein was detected on T cells, B cells and NK cells in B-hCD69 mice.