Basic Information

Strain name
C57BL/6-Cd70tm1(CD70)/Bcgen
Common name
B-hCD70 mice
Background
C57BL/6J
Catalog number
110770
NCBI Gene ID
Related genes
CD70, CD70 molecule, CD27L, LPFS3, CD27-L, CD27LG, TNFSF7, TNLG8A

Gene targeting strategy

The exons 1-3 of mouse Cd70 gene that encode the extracellular domain were replaced by human CD70 exons 1-3 in B-hCD70 mice.

mRNA expression analysis

Species-specific CD70 gene expression analysis in wild-type and humanized B-hCD70 mice by RT-PCR. Murine Cd70 mRNA was detected in thymocytes isolated from wild-type (+/+) mice, while human CD70 mRNA was detected in homozygous B-hCD70 (H/H) mice.

Protein expression analysis

Species-specific CD70 protein expression analysis in humanized B-hCD70 mice. Following LPS stimulation in vivo, bone marrow-derived dendritic cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD70 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD70 antibodies. Murine CD70 protein was detected in wild-type mice, while human CD70 protein was detected in B-hCD70 mice.