Basic Information

Strain name
Common name
B-hCD70 mice
Catalog number
Related genes
CD70, CD70 molecule, CD27L, LPFS3, CD27-L, CD27LG, TNFSF7, TNLG8A

Gene targeting strategy

Gene targeting strategy for B-hCD70 mice. The exons 1-3 of the mouse Cd70 gene that encode the extracellular domain were replaced by human CD70 exons 1-3 in B-hCD70 mice.

mRNA expression analysis

Strain specific analysis of CD70 gene expression in wild type (WT) mice and B-hCD70 mice by RT-PCR. Mouse CD70 mRNA was detectable only in thymocytes of WT mice (+/+) and B-hCD70 mice (H/+). Human CD70 mRNA was detectable only in homozygous B-hCD70 mice (H/+) but not in WT mice (+/+).

Protein expression analysis

Species-specific CD70 protein expression analysis in humanized B-hCD70 mice. Following LPS stimulation in vivo, bone marrow-derived dendritic cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD70 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD70 antibodies. Murine CD70 protein was detected in wild-type mice, while human CD70 protein was detected in B-hCD70 mice.

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