Basic Information

Strain name
C57BL/6-Cd70tm1(CD70)/Bcgen
Common name
B-hCD70 mice
Background
C57BL/6
Catalog number
110770
NCBI Gene ID
Related genes
CD70, CD70 molecule, CD27L, LPFS3, CD27-L, CD27LG, TNFSF7, TNLG8A

Targeting strategy

The exons 1-3 of mouse Cd70 gene that encode the extracellular domain were replaced by human CD70 exons 1-3 in B-hCD70 mice.

Details

mRNA expression analysis

Strain specific analysis of CD70 gene expression in wild type (WT) mice and B-hCD70 mice by RT-PCR.
Mouse Cd70 mRNA was detectable only in thymocytes of WT mice (+/+) and B-hCD70 mice (H/+). Human CD70 mRNA was detectable only in homozygous B-hCD70 mice (H/+) but not in WT mice (+/+).

Protein expression analysis-Dendritic cells

Strain specific CD70 expression analysis in homozygous B-hCD70 mice by flow cytometry.
Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCD70 mice (H/H) stimulated with LPS in vivo, and analyzed by flow cytometry with species-specific anti-CD70 antibody. Mouse CD70 was detectable in WT mice (+/+). Human CD70 was exclusively detectable in homozygous B-hCD70 mice (H/H) but not in WT mice (+/+).