Basic Information

Strain Name
C57BL/6-Nt5etm1(NT5E)Tnfrsf9tm1(TNFRSF9)/Bcgen
Common Name
B-hCD73/h4-1BB mice
Background
C57BL/6
Catalog number
111877
Aliases 
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA CDw137; CD137; ILA; TNFRSF9; 4-1BB
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD73/h4-1BB mice. The exons 2-7 of mouse Tnfrsf gene that encode the extracellular domain were replaced by human TNFRSF exons 2-7 in B-hCD73/h4-1BB mice. The full encoding region sequences of human NT5E gene was insert into mouse Nt5e in B-hCD73/h4-1BB mice.

Protein expression analysis

Strain specific CD73 expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse CD73 was only detectable in wild-type mice. Human CD73 was only detectable in homozygous B-hCD73/h4-1BB mice.

Strain specific CD73 expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse CD73 was only detectable in wild-type mice. Human CD73 was only detectable in homozygous B-hCD73/h4-1BB mice.

Strain specific CD73 expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse CD73 was only detectable in wild-type mice. Human CD73 was only detectable in homozygous B-hCD73/h4-1BB mice.

Strain specific 4-1BB expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse 4-1BB was only detectable in wild-type mice. Human 4-1BB was only detectable in homozygous B-hCD73/h4-1BB mice.

Strain specific 4-1BB expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse 4-1BB was only detectable in wild-type mice. Human 4-1BB was only detectable in homozygous B-hCD73/h4-1BB mice.

Strain specific 4-1BB expression analysis in wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD73/h4-1BB mice, stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.). Mouse 4-1BB was only detectable in wild-type mice. Human 4-1BB was only detectable in homozygous B-hCD73/h4-1BB mice.

Summary

Protein expression analysis:

Human CD73 and 4-1BB were exclusively detectable in homozygous B-hCD73/h4-1BB mice but not in wild type mice.

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