B-hCD73 mice plus

Basic Information

Strain Name
C57BL/6-Nt5etm2(NT5E)/Bcgen
Stock Number
110047
Common Name
B-hCD73 mice plus
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD73 (Nt5e, 5′ nucleotidase, ecto)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

CD73 is expressed on the surface of multiple cells of the immune system including T-cells, B-cells, NK-cells and myeloid-derived suppressor cells. CD73 catalyzes the final hydrolysis of AMP to adenosine following CD39 activity. Increased expression of CD73 has been associated with several tumor signaling pathways stimulated by hypoxyia in the TME. Anti-CD73 therapy in combination with both anti-PD1 and anti-CTLA4 therapy was shown to synergistically reduce syngeneic tumor burden and extend survival via IFNγ-dependent, Th1 CD4+-driven expansion of tumor-specific CD8+ T cells.

Details

Protein expression analysis

Strain specific CD73 expression analysis in heterozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD73 (H/+) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in heterozygous B-hCD73 and WT mice. Human CD73 was exclusively detectable in heterozygous B-hCD73 but not WT mice.

Strain specific CD73 expression analysis in heterozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD73 (H/+) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in heterozygous B-hCD73 and WT mice. Human CD73 was exclusively detectable in heterozygous B-hCD73 but not WT mice.

Strain specific CD73 expression analysis in heterozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD73 (H/+) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in heterozygous B-hCD73 and WT mice. Human CD73 was exclusively detectable in heterozygous B-hCD73 but not WT mice.

Strain specific CD73 expression analysis in homozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD73 (H/H) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in and WT mice. Human CD73 was exclusively detectable in homozygous B-hCD73 but not WT mice. MEDI9447 (in house) is a cross-reactivity antibody between mouse and human.

Strain specific CD73 expression analysis in homozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD73 (H/H) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in and WT mice. Human CD73 was exclusively detectable in homozygous B-hCD73 but not WT mice. MEDI9447 (in house) is a cross-reactivity antibody between mouse and human.

Strain specific CD73 expression analysis in homozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD73 (H/H) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in and WT mice. Human CD73 was exclusively detectable in homozygous B-hCD73 but not WT mice. MEDI9447 (in house) is a cross-reactivity antibody between mouse and human.

Strain specific CD73 expression analysis in homozygous B-hCD73 mice plus by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD73 (H/H) mice plus, and analyzed by flow cytometry with species-specific anti-CD73 antibody. Mouse CD73 was detectable in and WT mice. Human CD73 was exclusively detectable in homozygous B-hCD73 but not WT mice. MEDI9447 (in house) is a cross-reactivity antibody between mouse and human.

 

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