Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD79B mice. The exons 1~4 of mouse Cd79b gene that encode the extracellular domain were replaced by human CD79B exons 1~4 in B-hCD79B mice.
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Details
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Technical assessment of gene editing mouse model and existing models
Protein expression analysis
Strain specific CD79B expression analysis in heterozygous B-hCD79B mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD79B (H/+) mice, and analyzed by flow cytometry with species-specific CD79B antibody. Mouse CD79B was detectable in WT mice and heterozygous B-hCD79B. Human CD79B was exclusively detectable in heterozygous B-hCD79B but not WT mice.
mRNA expression analysis
Strain specific analysis of CD79B gene expression in WT and B-hCD79B mice by RT-PCR. Mouse Cd79b mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CD79B mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific CD79B expression analysis in homozygous B-hCD79B mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD79B (H/H) mice, and analyzed by flow cytometry with species-specific CD79B antibody. Mouse CD79B was detectable in WT mice and homozygous B-hCD79B due to the anti-mCD79B antibody is cross-reactive between mice and human. Human CD79B was exclusively detectable in homozygous B-hCD79B but not WT mice.
