Basic Information

Strain Name
C57BL/6-Cd79btm1(CD79B)/Bcgen
Stock Number
111096
Common Name
B-hCD79B mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD79B, AGM6, B29, IGB
Species
C57BL/6
Appearance
Black
Genotypes
homozygous
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD79B mice. The exons 1~4 of mouse Cd79b gene that encode the extracellular domain were replaced by human CD79B exons 1~4 in B-hCD79B mice.

Details

Technical assessment of gene editing mouse model and existing models

Protein expression analysis

Strain specific CD79B expression analysis in heterozygous B-hCD79B mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD79B (H/+) mice, and analyzed by flow cytometry with species-specific CD79B antibody. Mouse CD79B was detectable in WT mice and heterozygous B-hCD79B. Human CD79B was exclusively detectable in heterozygous B-hCD79B but not WT mice.

mRNA expression analysis

Strain specific analysis of CD79B gene expression in WT and B-hCD79B mice by RT-PCR. Mouse Cd79b mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CD79B mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis

Strain specific CD79B expression analysis in homozygous B-hCD79B mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD79B (H/H) mice, and analyzed by flow cytometry with species-specific CD79B antibody. Mouse CD79B was detectable in WT mice and homozygous B-hCD79B due to the anti-mCD79B antibody is cross-reactive between mice and human. Human CD79B was exclusively detectable in homozygous B-hCD79B but not WT mice.