Basic Information
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mRNA expression analysis
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Species-specific CD80 gene expression analysis in wild-type and humanized B-hCD80 mice by RT-PCR. Murine Cd80 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD80 mRNA was exclusively detected in homozygous B-hCD80 (H/H) mice.
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Protein expression analysis in macrophages
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Species-specific CD80 protein expression analysis in wild-type and humanized B-hCD80 mice. Peritoneal exudate macrophages (PEMs) were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD80 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD80 antibodies. Murine CD80 protein was detected in wild-type mice, while human CD80 protein was exclusively detected in homozygous B-hCD80 (H/H) mice.
Species-specific CD80 protein expression analysis in wild-type and humanized B-hCD80 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD80 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD80 antibodies. Murine CD80 protein was detected in wild-type mice, while human CD80 protein was exclusively detected in B-hCD80 mice.
