Basic Information

Strain name
C57BL/6-Cd80tm1(CD80)/Bcgen
Common Name
B-hCD80 mice
Background
C57BL/6J
Catalog number
110935
Related Genes
B7、BB1、B7-1、B7.1、LAB7、CD28LG、CD28LG1
NCBI Gene ID

mRNA expression analysis

Species-specific CD80 gene expression analysis in wild-type and humanized B-hCD80 mice by RT-PCR. Murine Cd80 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD80 mRNA was exclusively detected in homozygous B-hCD80 (H/H) mice.

Protein expression analysis in macrophages

Species-specific CD80 protein expression analysis in wild-type and humanized B-hCD80 mice. Peritoneal exudate macrophages (PEMs) were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD80 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD80 antibodies. Murine CD80 protein was detected in wild-type mice, while human CD80 protein was exclusively detected in homozygous B-hCD80 (H/H) mice.

Species-specific CD80 protein expression analysis in wild-type and humanized B-hCD80 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD80 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD80 antibodies. Murine CD80 protein was detected in wild-type mice, while human CD80 protein was exclusively detected in B-hCD80 mice.