Basic Information

Strain name
C57BL/6-Cd80tm1(CD80)/Bcgen
Common Name
B-hCD80 mice
Background
C57BL/6
Catalog number
110935
Related Genes
B7、BB1、B7-1、B7.1、LAB7、CD28LG、CD28LG1
NCBI Gene ID

mRNA expression analysis

Strain specific analysis of CD80 gene expression in wild type (WT) mice and B-hCD80 mice by RT-PCR. Mouse Cd80 mRNA was detectable only in splenocytes of WT mice (+/+). Human CD80 mRNA was detectable only in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).

Protein expression analysis in macrophages

Strain specific CD80 expression analysis in homozygous B-hCD80 mice by flow cytometry. Peritoneal exudative macrophages (PEMs) were collected from wild type (WT) mice (+/+) and homozygous B-hCD80 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD80 antibody. Mouse CD80 was detectable in WT mice (+/+). Human CD80 was exclusively detectable in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).

Strain specific CD80 expression analysis in homozygous B-hCD80 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD80 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD80 antibody. Mouse CD80 was detectable in WT mice (+/+). Human CD80 was exclusively detectable in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).