Basic Information
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Targeting strategy
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The human CD89 CDS was inserted into into the ROSA26 locus in B-hCD89 mice.
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mRNA expression analysis
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Strain specific analysis of CD89 gene expression in wild type (WT) mice and B-hCD89 mice by RT-PCR.
Human CD89 mRNA was detectable only in splenocytes of homozygous B-hCD89 mice (H/H) but not WT mice (+/+).
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Protein expression analysis
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Protein expression analysis in NK cells
Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.
Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in NK cells of homozygous B-hCD89 mice (H/H).
Protein expression analysis in CD11b+ cells
Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.
Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in CD11b+ cells of homozygous B-hCD89 mice (H/H).
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References
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1.Mkaddem S B, Rossato E, Heming N, et al. Anti-inflammatory role of the IgA Fc receptor (CD89): from autoimmunity to therapeutic perspectives[J]. Autoimmunity reviews, 2013, 12(6): 666-669.,
