Basic Information

Strain name
C57BL/6-Fcartm1(FCAR)/Bcgen
Common name
B-hCD89 mice
Catalog number
110864
Background
C57BL/6
NCBI Gene ID
Alias
CD89; FcalphaRI; CTB-61M7.2, FCAR, Fc fragment of IgA receptor

Targeting strategy

The human CD89 CDS was inserted into into the ROSA26 locus in B-hCD89 mice.

mRNA expression analysis

Strain specific analysis of CD89 gene expression in wild type (WT) mice and B-hCD89 mice by RT-PCR.

Human CD89 mRNA was detectable only in splenocytes of homozygous B-hCD89 mice (H/H) but not WT mice (+/+).

Protein expression analysis

Protein expression analysis in NK cells

Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.

Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in NK cells of homozygous B-hCD89 mice (H/H).

 

Protein expression analysis in CD11b+ cells

Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.

Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in CD11b+ cells of homozygous B-hCD89 mice (H/H).

References

1.Mkaddem S B, Rossato E, Heming N, et al. Anti-inflammatory role of the IgA Fc receptor (CD89): from autoimmunity to therapeutic perspectives[J]. Autoimmunity reviews, 2013, 12(6): 666-669.,