Basic Information

Strain name
C57BL/6-Fcartm1(FCAR)/Bcgen
Common name
B-hCD89 mice
Catalog number
110864
Background
C57BL/6
Alias
CD89; FcalphaRI; CTB-61M7.2, FCAR, Fc fragment of IgA receptor

Description

CD89, also known as FcαR, is a 55-100 kDa glycosylated protein. It belongs to the immunoglobulin gene family. It is expressed on granulocytes, monocytes, and macrophages but is absent on T cells. It can interact with IgA aggregates and plays an important role in IgA mediated immune responses. This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Targeting strategy

The human CD89 CDS was inserted into into the ROSA26 locus in B-hCD89 mice.

mRNA expression analysis

Strain specific analysis of CD89 gene expression in wild type (WT) mice and B-hCD89 mice by RT-PCR.

Human CD89 mRNA was detectable only in splenocytes of homozygous B-hCD89 mice (H/H) but not WT mice (+/+).

Protein expression analysis

Protein expression analysis in NK cells

Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.

Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in NK cells of homozygous B-hCD89 mice (H/H).

 

Protein expression analysis in CD11b+ cells

Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry.

Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in CD11b+ cells of homozygous B-hCD89 mice (H/H).

References

1.Mkaddem S B, Rossato E, Heming N, et al. Anti-inflammatory role of the IgA Fc receptor (CD89): from autoimmunity to therapeutic perspectives[J]. Autoimmunity reviews, 2013, 12(6): 666-669.,