Basic Information

Strain Name
C57BL/6N-Cdh3tm1(CDH3)/Bcgen
Common Name
B-hCDH3 mice
Background
C57BL/6N
Catalog Number
111101
Aliases
CDH3: CDHP, HJMD, PCAD
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCDH3 mice.

The exons 4-13 of mouse Cdh3 gene that encode the extracellular domain were replaced by human CDH3 exons 4-13 in B-hCDH3 mice.

Protein expression analysis

Simple Western analysis of CDH3 protein expression in homozygous B-hCDH3 mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hCDH3 mice (H/H), and then analyzed by Simple Western with species-specific anti-CDH3 antibody. 1mg/mL total proteins were loaded for simple western analysis. CDH3 protein was detected in the lung, spleen,thymus gland and skin of wild-type C57BL/6 mice and homozygous B-hCDH3 mice,as the antibody is cross-recognize both human and mouse CDH3. Human CDH3 was detected in the lung, spleen,thymus gland and skin of homozygous B-hCDH3 mice.

mRNA expression analysis

Strain specific analysis of CDH3 mRNA expression in wild-type C57BL/6 mice and B-hCDH3 mice by RT-PCR. Thymus gland RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCDH3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CDH3 primers. Mouse Cdh3 mRNA was detectable only in wild-type C57BL/6 mice. Human CDH3 mRNA was detectable only in homozygous B-hCDH3 mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Cdh3 mRNA was detectable in the thymus gland of wild-type C57BL/6 mice. Human CDH3 mRNA was detectable in B-hCDH3 mice.

Protein expression analysis:

Human CDH3 was only detected in the lung, spleen, thymus gland and skin of homozygous B-hCDH3 mice, and mouse CDH3 was detected in the lung, spleen, thymus gland and skin of wild-type C57BL/6 mice.

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