Basic Information

Strain name
C57BL/6-Clec9atm1(CLEC9A)/Bcgen
Common name
B-hCLEC9A mice
Background
C57BL/6N
Catalog number
111994
Aliases
CD370, DNGR-1, DNGR1, UNQ9341
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCLEC9A mice. The exons 2~6 of mouse Clec9a gene that encode the extracellular region were replaced by human CLEC9A exons 5~9 in B-hCLEC9A mice.

Protein expression analysis

Species-specific CLEC9A protein expression analysis in wild-type and humanized B-hCLEC9A mice. Splenic mCD8α+ DCs and mCD103+ DCs were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCLEC9A (H/+) mice and analyzed by flow cytometry using species-specific anti-CLEC9A antibodies. Murine CLEC9A protein was detected in wild-type and B-hCLEC9A mice, while human CLEC9A protein was detected in B-hCLEC9A mice.