Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCLEC9A mice. The exons 2~6 of mouse Clec9a gene that encode the extracellular region were replaced by human CLEC9A exons 5~9 in B-hCLEC9A mice.
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Protein expression analysis
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Strain specific CLEC9A expression analysis in homozygous B-hCELC9A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCLEC9A mice (H/H), and analyzed by flow cytometry with species-specific anti-CLEC9A antibody. Mouse CLEC9A was detectable in mCD8α+ DCs and mCD103+ DCs of wild-type mice. Human CLEC9A was exclusively detectable in mCD8α+ DCs and mCD103+ DCs of homozygous B-hCLEC9A mice but not in wild-type mice.
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Summary
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Protein expression analysis:
Mouse CLEC9A was detectable in mCD8α+ DCs and mCD103+ DCs of wild-type mice. Human CLEC9A was exclusively detectable in in mCD8α+ DCs and mCD103+ DCs of homozygous B-hCLEC9A mice but not in wild-type mice.
