The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity.The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. CSF1R(Colony-stimulating factor 1 receptor) , also known as CD115 and M-CSF-R, CSF1R belongs to the type Ⅲ protein tyrosine kinase receptor family is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. This gene product’s natural ligands include CSF1 and IL-34. CSF1R directly influences tissue macrophage and osteoclast differentiation and proliferation. CSF1R mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular.
Protein expression analysis
Strain specific CSF1 expression analysis in homozygous B-hCSF1/hCSF1R mice by ELISA. Splenocytes were collected from WT and homozygous B-hCSF1/hCSF1R (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific anti-CSF1 ELISA kit. Mouse CSF1 was detectable in WT. Human CSF1 was exclusively detectable in homozygous B-hCSF1/hCSF1R but not WT mice.
Strain specific CSF1R expression analysis in homozygous B-hCSF1/hCSF1R mice by flow cytometry. Blood were collected from WT and homozygous B-hCSF1/hCSF1R (H/H) mice, and analyzed by flow cytometry with species-specific anti-CSF1R antibody. Mouse CSF1R was detectable in WT .Human CSF1R was exclusively detectable in homozygous B-hCSF1/hCSF1R but not WT mice.