Basic Information
Description
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity.The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. CSF1R(Colony-stimulating factor 1 receptor) , also known as CD115 and M-CSF-R, CSF1R belongs to the type Ⅲ protein tyrosine kinase receptor family is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. This gene product’s natural ligands include CSF1 and IL-34. CSF1R directly influences tissue macrophage and osteoclast differentiation and proliferation. CSF1R mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular.
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Details
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Protein expression analysis
Strain specific CSF1 expression analysis in homozygous B-hCSF1/hCSF1R mice by ELISA. Splenocytes were collected from WT and homozygous B-hCSF1/hCSF1R (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific anti-CSF1 ELISA kit. Mouse CSF1 was detectable in WT. Human CSF1 was exclusively detectable in homozygous B-hCSF1/hCSF1R but not WT mice.
Strain specific CSF1R expression analysis in homozygous B-hCSF1/hCSF1R mice by flow cytometry. Blood were collected from WT and homozygous B-hCSF1/hCSF1R (H/H) mice, and analyzed by flow cytometry with species-specific anti-CSF1R antibody. Mouse CSF1R was detectable in WT .Human CSF1R was exclusively detectable in homozygous B-hCSF1/hCSF1R but not WT mice.
