Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCTLA4/hIL2RA mice. Exons 2-6 of mouse Il2ra gene that encode the extracellular domain were replaced by human IL2RA exons 2-6 in B-hCTLA4/hIL2RA mice. The exon 2 of mouse Ctla4 gene that encode the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hIL2RA mice.
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Protein expression analysis
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Species-specific CTLA4 and IL2RA protein expression analyses in wild-type and B-hCTLA4/hIL2RA mice. Following anti-mCD3ε stimulation in vivo, splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCTLA4/hIL2RA (H/H) mice, and analyzed by flow cytometry using species-specific anti-CTLA4 and anti-IL2RA antibodies. Mouse CTLA4 and IL2RA proteins were detected in wild-type mice, while human CTLA4 and IL2RA proteins were detected in B-hCTLA4/hIL2RA mice.
