Basic Information

Strain Name
C57BL/6-Ctla4tm1(CTLA4) Lag3tm1(LAG3)/Bcgen
Stock Number
120529
Common Name
B-hCTLA4/hLAG3 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Ctla4 (cytotoxic T-lymphocyte-associated protein 4); Lag3 (lymphocyte-activation gene 3)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Targeting Strategy

 

Gene targeting strategy for B-hCTLA4/hLAG3 mice. The exon 2 of mouse Ctla4 gene that  encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hLAG3 mice. The exons 2-3 of mouse Lag3 gene that  encode the extracellular domain were replaced by human LAG3 exons 2-3 in B-hCTLA4/hLAG3 mice.

Detail

Phenotype

Protein Expression Analysis

Strain specific CTLA4 and LAG3 expression analysis in homozygous B-hCTLA4/hLAG3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCTLA4/hLAG3 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CTLA4 and anti-LAG3 antibody. Mouse CTLA4 was detectable in WT mice, mouse LAG3 was detectable in homozygous B-hCTLA4/hLAG3 and WT mice due to the anti-mouse LAG3 antibody cross-reacts with human LAG3. Human CTLA4 and LAG3 were exclusively detectable in homozygous B-hCTLA4/hLAG3 but not WT mice.

References

  1. Cell Research (2018) 0:1–15; doi: 10.1038/s41422-018-0012-z
  2. Cancer Immunity (22 January 2013) Vol. 13, p. 5
  3. Journal of Immunological Methods, 18 November 2016. doi: 10.1016/j.jim.2017.04.006
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