Basic Information

Strain Name
C57BL/6-Ddr1tm1(DDR1)/Bcgen
Common Name
B-hDDR1 mice
Background
C57BL/6N
Catalog number
111121
Aliases
CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hDDR1 mice. Exons 3-12 of mouse Ddr1 gene that encode the extracellular sequences were replaced by human DDR1 exons 2-11 in B-hDDR1 mice.

mRNA expression analysis

Species-specific DDR1 gene expression analysis in wild-type and humanized B-hDDR1 mice by RT-PCR. Murine Ddr1 mRNA was detected brain tissue isolated from wild-type C57BL/6 (+/+) mice, while human DDR1 mRNA was detected in homozygous B-hDDR1 (H/H) mice.

Protein expression analysis

Species-specific DDR1 protein expression analysis in wild-type and humanized B-hDDR1 mice. Brain tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hDDR1 (H/H) mice, and analyzed by western blot using anti-DDR1 antibodies. Human DDR1 protein was detected in B-hDDR1 mice using an anti-human DDR1 specific antibody.

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