CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hDDR1 mice. Exons 3-12 of mouse Ddr1 gene that encode the extracellular sequences were replaced by human DDR1 exons 2-11 in B-hDDR1 mice.
mRNA expression analysis
Species-specific DDR1 gene expression analysis in wild-type and humanized B-hDDR1 mice by RT-PCR. Murine Ddr1 mRNA was detected brain tissue isolated from wild-type C57BL/6 (+/+) mice, while human DDR1 mRNA was detected in homozygous B-hDDR1 (H/H) mice.
Protein expression analysis
Species-specific DDR1 protein expression analysis in wild-type and humanized B-hDDR1 mice. Brain tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hDDR1 (H/H) mice, and analyzed by western blot using anti-DDR1 antibodies. Human DDR1 protein was detected in B-hDDR1 mice using an anti-human DDR1 specific antibody.